Nucleofector technology stretching transfection dimensions. Lonza group is a swiss multinational, chemicals and biotechnology company, headquartered in basel, with major facilities in europe, north america and south asia. Sf cell line 4dnucleofector x kit s from lonza biocompare. Recommended kit ssf cell line 4d nucleofector x kit amaxa 4dnucleofector protocol for hek293 atcc for 4d nucleofector x unittransfection in suspension human embryonal kidney, also known as hek293. For finetuning after the optimization process, please contact our scientific support teams. Amaxa 4dnucleofector protocol for stimulated human t cells. High efficiency electroporation deliver to hard to transfect cell lines and. View detailed import data, price, monthly trends, major importing countries, major ports of lonza. Recommended kitscell line optimization 4dnucleofector x kit. High efficiency electroporation of hard to transfect cell lines, stem cells and. Cell culture assays provide a means of quantitatively analyzing the presence, amount, or. Manufacturer specifications amaxa 4dnucleofector system, lonza.
Lonza s nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells. Amaxa 4dnucleofector optimization protocol for cell lines. At cambridge scientific products, we offer an extensive range of equipment for your laboratory. The nucleofector device delivers unique electrical parameters. In case no data are shown for the selected nucleofector platform, please take a look at our optimization strategy to get further. Are amaxa nucleofector kits somehow interchangeble neet to.
The nucleofector ii device with kit components as part of the nucleofector technology, lonza provides celltype specific amaxa optimized protocols for many different cell lines and primary cells. It is widely understood that primary cells are a more biologically and physiologically relevant in vitro tool than cell lines for studying human and animal biology. Ingenio cuvettes for the ezporator electroporation system and lonzaamaxa nucleofector ii2b devices ingenio cuvettes for the ezporator electroporation system and all conventional electroporators such as biorad and harvardbtx ingenio cell droppers. It belongs to bioresearch transfection services and produtcs category.
Lonza has announced the launch of its new 4d nucleofector lv unit, the latest addition to its transfection platform offering. Ingenio electroporation kit for lonzaamaxa nucleofector ii2b devices 0. We recently bought a 4d nucleofector, has anyone had any. The main drawback of the technique is the low transfection efficiency achieved when it is used for primary cell transfection, stem cell transfection and when working with suspension cell lines mainly due to the fact that it can be cytotoxic and relies on cell division for success. Do any of you use a lonza amaxa nucleofector setup. However, for decades, cell lines have been used as a standard tool. Human bone marrowderived mesenchymal stem cells hmscs are useful because they can replicate as undifferentiated cells and turn into bone, cartilage, fat, muscle, tendon and marrow stroma. Premature termination codon readthrough upregulates. Vca1001 size reactions 25 cell line nucleofector solution r 2. You can get a general idea about the specific program from lonza. Used lonza amaxa nucleofector ii 3012956 electroporation. The cell line nucleofector kit v is one of our five nucleofector kits suited for. The lv unit allows for closed, scalable transfection of larger cell numbers in the range of 1x10 7 to 1x10 9 cells. The electrical settings are preprogrammed for each optimized cell type and can be selected via the device or pc software.
Lonza walkersville p3 primary cell 4dnucleofector x kit l, 24 reactions, 100ul nucleocuvette vessels manufacturer. The cell line nucleofectortm kit lis for transfection of cell lines, e. The cell line nucleofectortm kit v is for transfection of cell lines, e. Lonza launches 4d nucleofector lv unit for largescale transfection 15 june 2016 scientific company news. The sf cell line 4d nucleofector tm x kit l is one of our three 4d nucleofector tm kits that is suited for transfecting difficulttotransfect cell lines in the 100 l nucleocuvette tm vessel format when working with the 4d nucleofector tm x unit. Recommended kitscell line optimization 4dnucleofector x kit amaxa 4dnucleofector optimization protocol for cell lines for 4dnucleofector x unittransfection in suspension for use with plasmid dna andor sirna note the cell line optimization protocol enables you to optimize 4dnucleofection conditions for a cell line of. Additionally, there are different kits out there, as well as amaxa nucleofection protocols that. It includes 4dnucleofector system, 96well shuttle system, ht nucleofector system, nucleofector 2b device. Lonza walkersville p3 primary cell 4d nucleofector x kit l, 24 reactions, 100ul nucleocuvette vessels manufacturer. The amaxa nucleofector technology is covered by patent. If no optimized protocol for the cell line of interest is available, we offer a cell line optimization nucleofector kit enabling you to easily establish the nucleofector technology for your specific cell line. Nucleofector supplement is added to the nucleofector solution it is stable for three months at 4c.
Bioresearch 4dnucleofector protocol for baf3 cells 3 note as leaving cells in nucleofector solution for extended periods of time may lead to reduced transfection efficiency and viability it is important. Ingenio electroporation solution competitive comparison. Amaxa cell line nucleofector kit r for nih3t3 dsmz acc59, cryopreserved nih swiss mouse embryo. We recently bought a 4d nucleofector, has anyone had any luck making the buffers themselves and reusing the cuvettesstrips these kits are pricey. Our solution is an improved electroporation technology, the nucleofector tm technology, originally introduced into the market by legacy amaxa in 2001. The table below shows data for the cell type and nucleofector platform selected. The acquisition of amaxa is in line with lonzas strategy of growing its lifescience platform. View the cells from where you are via the cytosmart tm app. Lonza has expended its stem cell portfolio to offer hmscs for translational and cell therapy research. Lonza 4dnucleofector nucleofector used miscellaneous. Nucleofection is a method to transfer substrates into mammalian cells so far considered difficult or even impossible to transfect.
Amaxa cell line nucleofector kit l lonza bioscience. Nhbe nucleofector kits discontinued by 31 dec 2011. Other product companynames mentioned herein respectiveowners. Pbmc should be purified from fresh human blood samples treated with an anticoagulant or from leukocyteenriched buffy coat. By visiting our website or using services provided by lonza. Lonza walkersville v4xp3024 the p3 primary cell 4dnucleofector x kit l is one of five 4dnucleofector kits suited for transfecting primary cells in the 100 l nucleocuvettetm vessel format when working with the 4dnucleofectortm. Interact with our search tool below to explore our current products, or filter by a product category to see our most up to date product offerings in each of these categories. Recommended kit s sf cell line 4d nucleofector lv kit cat no. Product overview the cell line nucleofector tm kit v is one of our five nucleofector tm kits suited for transfecting cell lines in the nucleofector tm 2b device. Does anyone have a homemade recipe for amaxa nucleofector kit 2. Who knows the difference between these 2 cell lines. Efficient gene transfer into the human natural killer cell.
Cells were resuspended in solution from nucleofector kit v, also available as part of the amaxa cell optimization kit, following the amaxa guidelines for cell line transfection see amaxa literature for further details about this kit. Transfecting human neural stem cells with the amaxa nucleofector. For these clones we recommend to follow the specific culturing recommendations of the respective supplier. Ingenio electroporation solution and kits mirus bio llc. Lonza walkersville p3 primary cell 4dnucleofector x kit l. Cell line optimization nucleofector tm kit for nucleofector tm device for determination of the optimal transfection conditions for any difficulttotransfect cell line that lacks an optimized protocol for the nucleofector tm iii2b device. Lonza 4d nucleofector core unit w x and y unit nucleofector.
Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population. Lonza biologics inc offers wide range of pharma products and services which includes nucleofector devices. Cell based assays and analysis are vital experimental tools in life science research and biomanufacturing. They are based on cell culture methods, where live cells are grown in vitro and used as model systems to assess the biochemistry and physiology of both healthy and diseased cells. Due to this independency from cell proliferation, it allows for efficient transfection of even nondividing primary cells like resting t cells or neurons and also speeds up expression. The nucleofector ii device with kit components as part of the nucleofector technology, lonza provides cell type specific amaxa optimized protocols for many different cell lines and primary cells. Amaxa cell line nucleofector kit v lonza bioscience. Nkl cells were transfected using the amaxa nucleofection technology amaxa, koeln, germany. It enables highly efficient, transfection of primary cells, stem cells, neurons, and cell lines that have traditionally been difficult to transfect via electroporation and other nonviral.
To speak with a representative, please fill out the contact form below or call us directly. Nucleofector kit v, 10 reactions vaca1003 szaboscandic. Pbmcs purified from fresh human blood samples treated with anticoagulant or from leucocyte rich buffy coat. Examples for such substrates are nucleic acids, like the dna of an isolated gene cloned into a plasmid, or small interfering rna sirna for knocking down expression of a specific endogenous gene. Product overview the cell line optimization 4d nucleofector tm x kit is the ideal tool to conveniently and rapidly determine nucleofection conditions for cell lines lacking an optimized protocol for the 4d nucleofector tm x unit with the unique capability of the 4d nucleofector tm x unit to address individual wells of a 16well nucleocuvette tm strip with different programs, cell line. V4xc2002 v4xc2020 transfection volume 1 ml 1 20 ml size reaction 2 1 nucleofector solution 2. Handmade solutions for amaxa lonza nucleofector we have tried buffers to nucleofect different cell lines using the amaxa device. The company offers nucleofector technology, a transfection solution which enables the transfer of various substrates, such as dna, rna, or peptides into cells. This kit has been determined to be optimal for transfecting following cell types.
The nucleofector technology relies on the combination of a nucleofector device and cell specifi c nucleofector kits. I need to know the parameters for the protocols t016 and t001. P3 primary cell 4dnucleofectortm x kit l description for efficient transfection of specific primary cells, e. Enhanced nucleic acid delivery using conventional electroporation devices that provide. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Also, remove the cell dish with culture media from the incubator and place it in the tissue culture hood. This easytouse live cell imaging and monitoring system captures images and records timelapse videos of your cell cultures while they reside in the incubator. P815 cells were transfected with the cell line nucleofector kit t, program c005 and 2 g of pmaxgfp vector. Using the amaxa minipipette, transfer the cell dna mix into the amaxa transfection cuvette. Aug 10, 2015 lonzas nucleofector technology is an improved electroporation technology that can help you achieve transfection results never before possible in primary cells, stem cells and hard to.
Make sure you have 1 ml of prewarmed culture media for the next step. Lonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of human umbilical vein endothelial cells huvec cultured in the absence of additive vascular endothelial growth factor vegf. Pc12 cells were transfected with the cell line nucleofector kit v, program u029 and 2 g of pmaxgfp vector. For further information please contact our scientific support team. Transfection of the human natural killer cell line nkl using traditional. Amaxa, nucleofector, nucleofection, 4d nucleofector, nucleocuvette, maxgfp, lgm3, clonetics registeredtrademarks lonzagroup itsaliates. Largescale transfection using the 4d nucleofector tm lv unit experience the new functional unit for the 4d nucleofector tm system which expands our proven system to largerscale transfection. Those data are either based on lonza optimized protocols or on results shared from customers who performed an optimization based on our guidelines.
Lonza launches 4dnucleofector lv unit for largescale. The nucleofector tm technology uses a specific combination of optimized electrical parameters and cell typespecific solutions which enables transfer of a molecule directly into the cells nucleus. Download a detailed overview summarising the nuclefector kits which are or will be discontinued as well as the corresponding replacement products. Does anyone have a homemade recipe for amaxa nucleofector. Amaxa biosystems gmbh develops and commercializes gene transfer technologies and products. Home products molecular biology electroporation lonza amaxa nucleofector ii.
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